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Structural Basis of the High Affinity Interaction between the Alphavirus Nonstructural Protein-3 (nsP3) and the SH3 Domain of Amphiphysin-2

We show that a peptide from Chikungunya virus nsP3 protein spanning residues 1728–1744 binds the amphiphysin-2 (BIN1) Src homology-3 (SH3) domain with an unusually high affinity (K(d) 24 nm). Our NMR solution complex structure together with isothermal titration calorimetry data on several related vi...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Tossavainen, Helena, Aitio, Olli, Hellman, Maarit, Saksela, Kalle, Permi, Perttu
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4965578/
https://ncbi.nlm.nih.gov/pubmed/27268056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M116.732412
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