Cell-free methods to produce structurally intact mammalian membrane proteins

The crystal structures of four membrane proteins, from bacteria or a unicellular alga, have been solved with samples produced by cell-free protein synthesis. In this study, for mammalian membrane protein production, we established the precipitating and soluble membrane fragment methods: membrane pro...

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發表在:Sci Rep
Main Authors: Shinoda, Takehiro, Shinya, Naoko, Ito, Kaori, Ishizuka-Katsura, Yoshiko, Ohsawa, Noboru, Terada, Takaho, Hirata, Kunio, Kawano, Yoshiaki, Yamamoto, Masaki, Tomita, Taisuke, Ishibashi, Yohei, Hirabayashi, Yoshio, Kimura-Someya, Tomomi, Shirouzu, Mikako, Yokoyama, Shigeyuki
格式: Artigo
語言:Inglês
出版: Nature Publishing Group 2016
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4964339/
https://ncbi.nlm.nih.gov/pubmed/27465719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep30442
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