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Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion

There is often interest in dissecting the relative contributions of the N-glycans, O-glycans and glycosphingolipids (GSLs) in regulating complex biological traits like cell signaling, adhesion, development and metastasis. To address this, we developed a CRISPR-Cas9 toolkit to selectively truncate ea...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Stolfa, Gino, Mondal, Nandini, Zhu, Yuqi, Yu, Xinheng, Buffone, Alexander, Neelamegham, Sriram
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4960646/
https://ncbi.nlm.nih.gov/pubmed/27458028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep30392
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