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Complementation of Arginine Auxotrophy for Genetic Transformation of Coxiella burnetii by Use of a Defined Axenic Medium

Host cell-free (axenic) culture of Coxiella burnetii in acidified citrate cysteine medium-2 (ACCM-2) has provided important opportunities for investigating the biology of this naturally obligate intracellular pathogen and enabled the development of tools for genetic manipulation. However, ACCM-2 has...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Sandoz, Kelsi M., Beare, Paul A., Cockrell, Diane C., Heinzen, Robert A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4959063/
https://ncbi.nlm.nih.gov/pubmed/26969695
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00261-16
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