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Isolation from Animal Tissue and Genetic Transformation of Coxiella burnetii Are Facilitated by an Improved Axenic Growth Medium

We recently described acidified citrate cysteine medium (ACCM), which supports host cell-free (axenic) growth of Coxiella burnetii. After 6 days of incubation, greater than 3 logs of growth was achieved with the avirulent Nine Mile phase II (NMII) strain. Here, we describe modified ACCM and culture...

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Detalhes bibliográficos
Main Authors: Omsland, Anders, Beare, Paul A., Hill, Joshua, Cockrell, Diane C., Howe, Dale, Hansen, Bryan, Samuel, James E., Heinzen, Robert A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3127619/
https://ncbi.nlm.nih.gov/pubmed/21478315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02826-10
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