Functional reconstitution of the lysosomal sialic acid carrier into proteoliposomes.
The lysosomal carrier for the acidic monosaccharides sialic acid and glucuronic acid was solubilized from rat liver lysosomal membranes and reconstituted into phospholipid vesicles. Membrane proteins were extracted from lysosomal membranes with Triton X-100. Upon removal of the detergent by absorpti...
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1992
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49551/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1631163/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.14.6609 |
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