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Functional reconstitution of the lysosomal sialic acid carrier into proteoliposomes.

The lysosomal carrier for the acidic monosaccharides sialic acid and glucuronic acid was solubilized from rat liver lysosomal membranes and reconstituted into phospholipid vesicles. Membrane proteins were extracted from lysosomal membranes with Triton X-100. Upon removal of the detergent by absorpti...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Mancini, G M, Beerens, C E, Galjaard, H, Verheijen, F W
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49551/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1631163/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.14.6609
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