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Derivation, expansion and differentiation of induced pluripotent stem cells in continuous suspension cultures

We demonstrate derivation of induced pluripotent stem cells (iPSCs) from terminally differentiated mouse cells in serum- and feeder-free stirred suspension cultures. Temporal analysis of global gene expression revealed high correlations between cells reprogrammed in suspension and cells reprogrammed...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Fluri, David A., Tonge, Peter D., Song, Hannah, Baptista, Ricardo P., Shakiba, Nika, Shukla, Shreya, Clarke, Geoffrey, Nagy, Andras, Zandstra, Peter W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4954777/
https://ncbi.nlm.nih.gov/pubmed/22447133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1939
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