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Time-resolved RNA SHAPE chemistry: quantitative RNA structure analysis in one second snapshots and at single nucleotide resolution
RNA SHAPE chemistry exploits the discovery that conformationally dynamic nucleotides preferentially adopt conformations that facilitate reaction between the 2′-OH group and a hydroxyl-selective electrophile, such as benzoyl cyanide (BzCN), to form a 2′-O-adduct. BzCN is ideally suited for quantitati...
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| 發表在: | Nat Protoc |
|---|---|
| Main Authors: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2009
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4950915/ https://ncbi.nlm.nih.gov/pubmed/19745823 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.126 |
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