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A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization

The human embryonic kidney 293 (HEK-293) cells are commonly used as host for the heterologous expression of membrane proteins not least because they have a high transfection efficiency and faithfully translate and process proteins. In addition, their cell size, morphology and division rate, and low...

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Détails bibliographiques
Publié dans:Front Physiol
Auteurs principaux: Ooi, Amanda, Wong, Aloysius, Esau, Luke, Lemtiri-Chlieh, Fouad, Gehring, Chris
Format: Artigo
Langue:Inglês
Publié: Frontiers Media S.A. 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4949579/
https://ncbi.nlm.nih.gov/pubmed/27486406
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fphys.2016.00300
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