Llwytho...

Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster

Transformation-associated recombination (TAR) in yeast is a rapid and inexpensive method for cloning and assembly of large DNA fragments, which relies on natural homologous recombination. Two vectors, based on p15a and F-factor replicons that can be maintained in yeast, E. coli and streptomycetes ha...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:PLoS One
Prif Awduron: Bilyk, Oksana, Sekurova, Olga N., Zotchev, Sergey B., Luzhetskyy, Andriy
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science 2016
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4943663/
https://ncbi.nlm.nih.gov/pubmed/27410036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0158682
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