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Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster
Transformation-associated recombination (TAR) in yeast is a rapid and inexpensive method for cloning and assembly of large DNA fragments, which relies on natural homologous recombination. Two vectors, based on p15a and F-factor replicons that can be maintained in yeast, E. coli and streptomycetes ha...
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| Publicado no: | PLoS One |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4943663/ https://ncbi.nlm.nih.gov/pubmed/27410036 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0158682 |
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