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Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Regulation of protein abundance is crucial to virtually every cellular process. Protein abundance reflects the integration of the rates of protein synthesis and protein degradation. Many assays reporting on protein abundance (e.g., single-time point western blotting, flow cytometry, fluorescence mic...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Buchanan, Bryce W., Lloyd, Michael E., Engle, Sarah M., Rubenstein, Eric M.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941941/
https://ncbi.nlm.nih.gov/pubmed/27167179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53975
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