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Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Regulation of protein abundance is crucial to virtually every cellular process. Protein abundance reflects the integration of the rates of protein synthesis and protein degradation. Many assays reporting on protein abundance (e.g., single-time point western blotting, flow cytometry, fluorescence mic...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Buchanan, Bryce W., Lloyd, Michael E., Engle, Sarah M., Rubenstein, Eric M.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941941/
https://ncbi.nlm.nih.gov/pubmed/27167179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53975
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