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Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Regulation of protein abundance is crucial to virtually every cellular process. Protein abundance reflects the integration of the rates of protein synthesis and protein degradation. Many assays reporting on protein abundance (e.g., single-time point western blotting, flow cytometry, fluorescence mic...

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteurs: Buchanan, Bryce W., Lloyd, Michael E., Engle, Sarah M., Rubenstein, Eric M.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2016
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941941/
https://ncbi.nlm.nih.gov/pubmed/27167179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53975
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