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Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography

The norovirus capsid is composed of a single major structural protein, termed VP1. VP1 is subdivided into a shell (S) domain and a protruding (P) domain. The S domain forms a contiguous scaffold around the viral RNA, whereas the P domain forms viral spikes on the S domain and contains determinants f...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Leuthold, Mila M., Koromyslova, Anna D., Singh, Bishal K., Hansman, Grant S.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941939/
https://ncbi.nlm.nih.gov/pubmed/27167457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53845
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