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An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation
The plasmid vector pGreenII is widely used to produce plant transformants via a process that involves propagation in Escherichia coli. However, we show here that pGreenII-based constructs can be unstable in E. coli as a consequence of them hampering cell division and promoting cell death. In additio...
Uloženo v:
| Vydáno v: | G3 (Bethesda) |
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| Hlavní autoři: | , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Genetics Society of America
2016
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4938672/ https://ncbi.nlm.nih.gov/pubmed/27194805 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.116.029405 |
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