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An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation

The plasmid vector pGreenII is widely used to produce plant transformants via a process that involves propagation in Escherichia coli. However, we show here that pGreenII-based constructs can be unstable in E. coli as a consequence of them hampering cell division and promoting cell death. In additio...

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Podrobná bibliografie
Vydáno v:G3 (Bethesda)
Hlavní autoři: Watson, Michael R., Lin, Yu-fei, Hollwey, Elizabeth, Dodds, Rachel E., Meyer, Peter, McDowall, Kenneth J.
Médium: Artigo
Jazyk:Inglês
Vydáno: Genetics Society of America 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4938672/
https://ncbi.nlm.nih.gov/pubmed/27194805
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.116.029405
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