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Plant Transformation by Coinoculation with a Disarmed Agrobacterium tumefaciens Strain and an Escherichia coli Strain Carrying Mobilizable Transgenes

Transformation of Nicotiana tabacum leaf explants was attempted with Escherichia coli as a DNA donor either alone or in combination with Agrobacterium tumefaciens. We constructed E. coli donor strains harboring either the promiscuous IncP-type or IncN-type conjugal transfer system and second plasmid...

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Detalhes bibliográficos
Main Authors: Pappas, Katherine M., Winans, Stephen C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC262305/
https://ncbi.nlm.nih.gov/pubmed/14602634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.11.6731-6739.2003
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