Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants

Genetic screening using random transposon insertions has been a powerful tool for uncovering biology in prokaryotes, where whole-genome saturating screens have been performed in multiple organisms. In eukaryotes, such screens have proven more problematic, in part because of the lack of a sensitive a...

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Vydáno v:Genome Res
Hlavní autoři: Bronner, Iraad F., Otto, Thomas D., Zhang, Min, Udenze, Kenneth, Wang, Chengqi, Quail, Michael A., Jiang, Rays H.Y., Adams, John H., Rayner, Julian C.
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2016
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Method
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4937560/
https://ncbi.nlm.nih.gov/pubmed/27197223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.200279.115
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