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Quantification of designer nuclease induced mutation rates: a direct comparison of different methods

Designer nucleases are broadly applied to induce site-specific DNA double-strand breaks (DSB) in genomic DNA. These are repaired by nonhomologous end joining leading to insertions or deletions (in/dels) at the respective DNA-locus. To detect in/del mutations, the heteroduplex based T7-endonuclease I...

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Vydáno v:Mol Ther Methods Clin Dev
Hlavní autoři: Ehrke-Schulz, Eric, Bergmann, Thorsten, Schiwon, Maren, Doerner, Johannes, Saydaminova, Kamola, Lieber, Andre, Ehrhardt, Anja
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4934480/
https://ncbi.nlm.nih.gov/pubmed/27419195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtm.2016.47
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