Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

BACKGROUND: The success of the CRISPR/Cas9 genome editing technique depends on the choice of the guide RNA sequence, which is facilitated by various websites. Despite the importance and popularity of these algorithms, it is unclear to which extent their predictions are in agreement with actual measu...

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Bibliographic Details
Published in:Genome Biol
Main Authors: Haeussler, Maximilian, Schönig, Kai, Eckert, Hélène, Eschstruth, Alexis, Mianné, Joffrey, Renaud, Jean-Baptiste, Schneider-Maunoury, Sylvie, Shkumatava, Alena, Teboul, Lydia, Kent, Jim, Joly, Jean-Stephane, Concordet, Jean-Paul
Format: Artigo
Language:Inglês
Published: BioMed Central 2016
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Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4934014/
https://ncbi.nlm.nih.gov/pubmed/27380939
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-1012-2
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