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Purification of a distinctive form of endotoxin-induced nitric oxide synthase from rat liver.

An endotoxin-induced form of nitric oxide synthase (EC 1.14.23) was purified to homogeneity from rat liver by sequential anion-exchange chromatography and affinity chromatography using 2',5'-ADP-Sepharose. The enzyme has a subunit molecular mass of 135 kDa as determined by SDS/PAGE, a maximum specif...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Evans, T, Carpenter, A, Cohen, J
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49291/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1376917/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.12.5361
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