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Purification of a distinctive form of endotoxin-induced nitric oxide synthase from rat liver.

An endotoxin-induced form of nitric oxide synthase (EC 1.14.23) was purified to homogeneity from rat liver by sequential anion-exchange chromatography and affinity chromatography using 2',5'-ADP-Sepharose. The enzyme has a subunit molecular mass of 135 kDa as determined by SDS/PAGE, a maxi...

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Detalhes bibliográficos
Main Authors: Evans, T, Carpenter, A, Cohen, J
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC49291/
https://ncbi.nlm.nih.gov/pubmed/1376917
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