Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Nucleosomes are the smallest structural unit of chromatin, composed of 147 base pairs of DNA wrapped around an octamer of histone proteins. Histone function is mediated by extensive post-translational modification by a myriad of nuclear proteins. These modifications are critical for nuclear integrit...

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteurs: Sidoli, Simone, Bhanu, Natarajan V., Karch, Kelly R., Wang, Xiaoshi, Garcia, Benjamin A.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2016
Onderwerpen:
Biochemistry
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4927705/
https://ncbi.nlm.nih.gov/pubmed/27286567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54112
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