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Fluorescence Spectroscopy in Thermodynamic and Kinetic Analysis of pH-Dependent Membrane Protein Insertion

Experimental determination of the free energy stabilizing the structure of membrane proteins in their native lipid environment is undermined by a lack of appropriate methods and suitable model systems. Here, we demonstrate how fluorescence correlation spectroscopy can be used to characterize thermod...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Autor principal: Ladokhin, Alexey S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4922797/
https://ncbi.nlm.nih.gov/pubmed/21609856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(09)66002-X
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