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Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

In this article, we describe a detailed protocol for miRNA detection in breast cancer tissue using in situ hybridization with a digoxigenin-labelled LNA (Locked Nucleic Acid) probe. The probe was recognized by anti-DIG alkaline phosphatase antibodies and later developed using alkaline phosphatase su...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Patel, Yogin, Lee, Ji S., Chen, Hexin
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4922489/
https://ncbi.nlm.nih.gov/pubmed/27341462
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53928
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