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A scalable strategy for high-throughput GFP tagging of endogenous human proteins

A central challenge of the postgenomic era is to comprehensively characterize the cellular role of the ∼20,000 proteins encoded in the human genome. To systematically study protein function in a native cellular background, libraries of human cell lines expressing proteins tagged with a functional se...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Leonetti, Manuel D., Sekine, Sayaka, Kamiyama, Daichi, Weissman, Jonathan S., Huang, Bo
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4922190/
https://ncbi.nlm.nih.gov/pubmed/27274053
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1606731113
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