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The Outer Membrane of Gram-negative Bacteria: Lipid A Isolation and Characterization

The isolation and characterization of the lipid A domain of lipopolysaccharide (LPS) are important methodologies utilized to gain understanding of the Gram-negative cell envelope. Here, we describe protocols often employed by our laboratory for small- and large-scale isolation of lipid A from bacter...

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Publicado en:Methods Mol Biol
Main Authors: Hankins, Jessica V., Madsen, James A., Needham, Brittany D., Brodbelt, Jennifer S., Trent, M. Stephen
Formato: Artigo
Idioma:Inglês
Publicado: 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4919815/
https://ncbi.nlm.nih.gov/pubmed/23299739
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-245-2_15
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