Optimization of Quantitative PCR Methods for Enteropathogen Detection

Detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. Here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficien...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Liu, Jie, Gratz, Jean, Amour, Caroline, Nshama, Rosemary, Walongo, Thomas, Maro, Athanasia, Mduma, Esto, Platts-Mills, James, Boisen, Nadia, Nataro, James, Haverstick, Doris M., Kabir, Furqan, Lertsethtakarn, Paphavee, Silapong, Sasikorn, Jeamwattanalert, Pimmada, Bodhidatta, Ladaporn, Mason, Carl, Begum, Sharmin, Haque, Rashidul, Praharaj, Ira, Kang, Gagandeep, Houpt, Eric R.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4918952/
https://ncbi.nlm.nih.gov/pubmed/27336160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0158199
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