Optimization of Quantitative PCR Methods for Enteropathogen Detection

Detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. Here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficien...

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Publicado en:PLoS One
Main Authors: Liu, Jie, Gratz, Jean, Amour, Caroline, Nshama, Rosemary, Walongo, Thomas, Maro, Athanasia, Mduma, Esto, Platts-Mills, James, Boisen, Nadia, Nataro, James, Haverstick, Doris M., Kabir, Furqan, Lertsethtakarn, Paphavee, Silapong, Sasikorn, Jeamwattanalert, Pimmada, Bodhidatta, Ladaporn, Mason, Carl, Begum, Sharmin, Haque, Rashidul, Praharaj, Ira, Kang, Gagandeep, Houpt, Eric R.
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2016
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4918952/
https://ncbi.nlm.nih.gov/pubmed/27336160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0158199
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