Optimization of Quantitative PCR Methods for Enteropathogen Detection

Detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. Here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficien...

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Опубликовано в: :PLoS One
Главные авторы: Liu, Jie, Gratz, Jean, Amour, Caroline, Nshama, Rosemary, Walongo, Thomas, Maro, Athanasia, Mduma, Esto, Platts-Mills, James, Boisen, Nadia, Nataro, James, Haverstick, Doris M., Kabir, Furqan, Lertsethtakarn, Paphavee, Silapong, Sasikorn, Jeamwattanalert, Pimmada, Bodhidatta, Ladaporn, Mason, Carl, Begum, Sharmin, Haque, Rashidul, Praharaj, Ira, Kang, Gagandeep, Houpt, Eric R.
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2016
Предметы:
Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4918952/
https://ncbi.nlm.nih.gov/pubmed/27336160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0158199
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