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Generating high accuracy peptide binding data in high throughput with yeast surface display and SORTCERY

Library methods are widely used to study protein-protein interactions, and high-throughput screening or selection followed by sequencing can identify a large number of peptide ligands for a protein target. In this chapter we describe a procedure called "SORTCERY" that can rank the affiniti...

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Veröffentlicht in:Methods Mol Biol
Hauptverfasser: Reich, Lothar “Luther”, Dutta, Sanjib, Keating, Amy E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4912380/
https://ncbi.nlm.nih.gov/pubmed/27094295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3569-7_14
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