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IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly
In vivo homologous recombination holds the potential for optimal molecular cloning, however, current strategies require specialised bacterial strains or laborious protocols. Here, we exploit a recA-independent recombination pathway, present in widespread laboratory E.coli strains, to develop IVA (In...
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| Publicado no: | Sci Rep |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4893743/ https://ncbi.nlm.nih.gov/pubmed/27264908 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep27459 |
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