Protein purification and cloning of diacylglycerol lipase from rat brain

Diacylglycerol (DG) lipase, which hydrolyses 1-stearoyl-2-arachidonyl-sn-glycerol to produce an endocannabinoid, 2-arachidonoylglycerol, was purified from the soluble fraction of rat brain lysates. DG lipase was purified about 1,200-fold by a sequential column chromatographic procedure. Among protei...

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Veröffentlicht in:J Biochem
Hauptverfasser: Aso, Chizu, Araki, Mari, Ohshima, Noriyasu, Tatei, Kazuaki, Hirano, Tohko, Obinata, Hideru, Kishi, Mikiko, Kishimoto, Koji, Konishi, Akimitsu, Goto, Fumio, Sugimoto, Hiroyuki, Izumi, Takashi
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2016
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Regular Papers
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4892392/
https://ncbi.nlm.nih.gov/pubmed/26790472
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jb/mvw002
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