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In Vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae

Delitto perfetto is a site-specific in vivo mutagenesis system that has been developed to generate changes at will in the genome of the yeast Saccharomyces cerevisiae. Using this technique, it is possible to rapidly and efficiently engineer yeast strains without requiring several intermediate steps...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Stuckey, Samantha, Mukherjee, Kuntal, Storici, Francesca
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4890625/
https://ncbi.nlm.nih.gov/pubmed/21660695
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-129-1_11
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