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Generation and purification of catalytically active recombinant SIRT5 protein

Sirtuin-family deacylases promote health and longevity in mammals. The sirtuin SIRT5 localizes predominantly to the mitochondrial matrix. SIRT5 preferentially removes negatively charged modifications from its target lysines: succinylation, malonylation, and glutarylation. It regulates protein substr...

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Bibliographic Details
Published in:Methods Mol Biol
Main Authors: Kumar, Surinder, Lombard, David B.
Format: Artigo
Language:Inglês
Published: 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4890613/
https://ncbi.nlm.nih.gov/pubmed/27246219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3667-0_16
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