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High density three-dimensional localization microscopy across large volumes

Extending three-dimensional (3D) single molecule localization microscopy away from the coverslip and into thicker specimens will greatly broaden its biological utility. However, localizing molecules in 3D with high precision in such samples, while simultaneously achieving the extreme labeling densit...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Legant, Wesley R., Shao, Lin, Grimm, Jonathan B., Brown, Timothy A., Milkie, Daniel E., Avants, Brian B., Lavis, Luke D., Betzig, Eric
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4889433/
https://ncbi.nlm.nih.gov/pubmed/26950745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3797
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