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Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer

We describe the use of “SuperSelective” primers that enable the detection and quantitation of somatic mutations whose presence relates to cancer diagnosis, prognosis, and therapy, in real-time PCR assays that can potentially analyze rare DNA fragments present in blood samples (liquid biopsies). The...

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Publicat a:PLoS One
Autors principals: Vargas, Diana Y., Kramer, Fred Russell, Tyagi, Sanjay, Marras, Salvatore A. E.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2016
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4887114/
https://ncbi.nlm.nih.gov/pubmed/27244445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0156546
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