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Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells

Real-time and noninvasive imaging of intracellular second messengers in mammalian cells, while preserving their in vivo phenotype, requires biosensors of exquisite constitution. Here we provide the methodology for utilizing the single wavelength cGMP-biosensor δ-FlincG in aortic vascular smooth musc...

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Bibliografski detalji
Izdano u:Methods Mol Biol
Glavni autori: Held, Kara F., Dostmann, Wolfgang R.
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4887092/
https://ncbi.nlm.nih.gov/pubmed/23709030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-459-3_8
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