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Chemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells

We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and 3 other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration in...

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Podrobná bibliografie
Vydáno v:J Am Chem Soc
Hlavní autoři: Xue, Min, Wei, Wei, Su, Yapeng, Kim, Jungwoo, Shin, Young Shik, Mai, Wilson X., Nathanson, David A., Heath, James R.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4883658/
https://ncbi.nlm.nih.gov/pubmed/25789560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.5b00944
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