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Chemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells

We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and 3 other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration in...

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Foilsithe in:J Am Chem Soc
Main Authors: Xue, Min, Wei, Wei, Su, Yapeng, Kim, Jungwoo, Shin, Young Shik, Mai, Wilson X., Nathanson, David A., Heath, James R.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4883658/
https://ncbi.nlm.nih.gov/pubmed/25789560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.5b00944
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