How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?

RNA-seq is now the technology of choice for genome-wide differential gene expression experiments, but it is not clear how many biological replicates are needed to ensure valid biological interpretation of the results or which statistical tools are best for analyzing the data. An RNA-seq experiment w...

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Tallennettuna:
Bibliografiset tiedot
Julkaisussa:RNA
Päätekijät: Schurch, Nicholas J., Schofield, Pietá, Gierliński, Marek, Cole, Christian, Sherstnev, Alexander, Singh, Vijender, Wrobel, Nicola, Gharbi, Karim, Simpson, Gordon G., Owen-Hughes, Tom, Blaxter, Mark, Barton, Geoffrey J.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Cold Spring Harbor Laboratory Press 2016
Aiheet:
Article
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4878611/
https://ncbi.nlm.nih.gov/pubmed/27022035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.053959.115
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