Integrated Microfluidic Isolation of Aptamers Using Electrophoretic Oligonucleotide Manipulation

We present a microfluidic approach to integrated isolation of DNA aptamers via systematic evolution of ligands by exponential enrichment (SELEX). The approach employs a microbead-based protocol for the processes of affinity selection and amplification of target-binding oligonucleotides, and an elect...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Kim, Jinho, Olsen, Timothy R., Zhu, Jing, Hilton, John P., Yang, Kyung-Ae, Pei, Renjun, Stojanovic, Milan N., Lin, Qiao
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group 2016
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4877600/
https://ncbi.nlm.nih.gov/pubmed/27217242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep26139
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