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Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

BACKGROUND: The classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although this approach is largely successful, it frequently delivers clones with unpredicted production characteristics...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Schwarzhans, Jan-Philipp, Wibberg, Daniel, Winkler, Anika, Luttermann, Tobias, Kalinowski, Jörn, Friehs, Karl
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4874018/
https://ncbi.nlm.nih.gov/pubmed/27206580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0486-7
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