EC-QCL mid-IR transmission spectroscopy for monitoring dynamic changes of protein secondary structure in aqueous solution on the example of β-aggregation in alcohol-denaturated α-chymotrypsin

In this work, a novel EC-QCL-based setup for mid-IR transmission measurements in the amide I region is introduced for monitoring dynamic changes in secondary structure of proteins. For this purpose, α-chymotrypsin (aCT) acts as a model protein, which gradually forms intermolecular β-sheet aggregates...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Anal Bioanal Chem
Egile Nagusiak: Alcaráz, Mirta R., Schwaighofer, Andreas, Goicoechea, Héctor, Lendl, Bernhard
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Springer Berlin Heidelberg 2016
Gaiak:
Research Paper
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4873525/
https://ncbi.nlm.nih.gov/pubmed/27007739
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-016-9464-5
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