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On the acquisition and analysis of microscale thermophoresis data

A comprehensive understanding of the molecular mechanisms underpinning cellular functions is dependent on a detailed characterization of the energetics of macromolecular binding, often quantified by the equilibrium dissociation constant, K(D). While many biophysical methods may be used to obtain K(D...

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Publicat a:Anal Biochem
Autors principals: Scheuermann, Thomas H., Padrick, Shae B., Gardner, Kevin H., Brautigam, Chad A.
Format: Artigo
Idioma:Inglês
Publicat: 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4873313/
https://ncbi.nlm.nih.gov/pubmed/26739938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.12.013
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