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Multi-signal sedimentation velocity analysis with mass conservation for determining the stoichiometry of protein complexes.

Multi-signal sedimentation velocity analytical ultracentrifugation (MSSV) is a powerful tool for the determination of the number, stoichiometry, and hydrodynamic shape of reversible protein complexes in two- and three-component systems. In this method, the evolution of sedimentation profiles of macr...

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Autors principals: Chad A Brautigam, Shae B Padrick, Peter Schuck
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science (PLoS) 2013-01-01
Col·lecció:PLoS ONE
Accés en línia:http://europepmc.org/articles/PMC3656001?pdf=render
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