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Overcoming the Refractory Expression of Secreted Recombinant Proteins in Mammalian Cells through Modification of the Signal Peptide and Adjacent Amino Acids

The expression and subsequent purification of mammalian recombinant proteins is of critical importance to many areas of biological science. To maintain the appropriate tertiary structure and post-translational modifications of such proteins, transient mammalian expression systems are often adopted....

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Bibliografiska uppgifter
I publikationen:PLoS One
Huvudupphovsmän: Güler-Gane, Gülin, Kidd, Sara, Sridharan, Sudharsan, Vaughan, Tristan J., Wilkinson, Trevor C. I., Tigue, Natalie J.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Public Library of Science 2016
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4873207/
https://ncbi.nlm.nih.gov/pubmed/27195765
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0155340
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