Isolation and Molecular Profiling of Primary Mouse Retinal Ganglion Cells: Comparison of Phenotypes from Healthy and Glaucomatous Retinas

Loss of functional retinal ganglion cells (RGC) is an element of retinal degeneration that is poorly understood. This is in part due to the lack of a reliable and validated protocol for the isolation of primary RGCs. Here we optimize a feasible, reproducible, standardized flow cytometry-based protoc...

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Detalhes bibliográficos
Publicado no:Front Aging Neurosci
Main Authors: Chintalapudi, Sumana R., Djenderedjian, Levon, Stiemke, Andrew B., Steinle, Jena J., Jablonski, Monica M., Morales-Tirado, Vanessa M.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2016
Assuntos:
Neuroscience
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4870266/
https://ncbi.nlm.nih.gov/pubmed/27242509
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fnagi.2016.00093
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