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Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader
Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ß-d-galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4865525/ https://ncbi.nlm.nih.gov/pubmed/27036618 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2016.03.017 |
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