Structural and biochemical characterisation of Archaeoglobus fulgidus esterase reveals a bound CoA molecule in the vicinity of the active site

A new carboxyl esterase, AF-Est2, from the hyperthermophilic archaeon Archaeoglobus fulgidus has been cloned, over-expressed in Escherichia coli and biochemically and structurally characterized. The enzyme has high activity towards short- to medium-chain p-nitrophenyl carboxylic esters with optimal...

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Vydáno v:Sci Rep
Hlavní autoři: Sayer, Christopher, Finnigan, William, Isupov, Michail N., Levisson, Mark, Kengen, Servé W. M., van der Oost, John, Harmer, Nicholas J., Littlechild, Jennifer A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2016
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4861933/
https://ncbi.nlm.nih.gov/pubmed/27160974
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25542
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