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Quantitating Fluorescence Intensity From Fluorophores: Practical Use of MESF Values

The present work uses fluorescein as the model fluorophore and points out critical steps in the use of MESF (Molecules of Equivalent Soluble Fluorophores) values for quantitative flow cytometric measurements. It has been found that emission spectrum matching between a reference solution and an analy...

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Bibliographic Details
Published in:J Res Natl Inst Stand Technol
Main Authors: Wang, Lili, Gaigalas, Adolfas K., Abbasi, Fatima, Marti, Gerald E., Vogt, Robert F., Schwartz, Abe
Format: Artigo
Language:Inglês
Published: [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology 2002
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4859266/
https://ncbi.nlm.nih.gov/pubmed/27446735
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.6028/jres.107.027
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